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zetaview nanoparticle tracking video microscope  (Particle Metrix)

 
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    Structured Review

    Particle Metrix zetaview nanoparticle tracking video microscope
    ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) <t>ZetaView</t> analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.
    Zetaview Nanoparticle Tracking Video Microscope, supplied by Particle Metrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zetaview+nanoparticle+tracking+video+microscope/pmc11691634-341-12-17?v=Particle+Metrix
    Average 90 stars, based on 1 article reviews
    zetaview nanoparticle tracking video microscope - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Blood-derived APLP1 + extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases"

    Article Title: Blood-derived APLP1 + extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases

    Journal: Science Advances

    doi: 10.1126/sciadv.ado6894

    ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) ZetaView analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.
    Figure Legend Snippet: ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) ZetaView analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.

    Techniques Used: Derivative Assay, Concentration Assay, Western Blot, Clinical Proteomics, Fluorescence, Immunostaining



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    ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) <t>ZetaView</t> analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.
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    ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) <t>ZetaView</t> analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.
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    ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) <t>ZetaView</t> analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.
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    ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) <t>ZetaView</t> analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.
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    ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) <t>ZetaView</t> analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.
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    Image Search Results


    ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) ZetaView analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.

    Journal: Science Advances

    Article Title: Blood-derived APLP1 + extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases

    doi: 10.1126/sciadv.ado6894

    Figure Lengend Snippet: ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) ZetaView analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.

    Article Snippet: EVs were diluted in PBS (1-ml final volume) and examined under a ZetaView Nanoparticle Tracking Video Microscope (Particle Metrix, Inning, Germany).

    Techniques: Derivative Assay, Concentration Assay, Western Blot, Clinical Proteomics, Fluorescence, Immunostaining